Coding

Part:BBa_K929100

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-24)

Anti-EGFR scFv425

General information

Anti-EGFR scFv425
UP12 BBa K929100.png
BioBrick Nr. BBa_929100
RFC standard RFC 25
Requirement pSB1C3
Source plasmid pKMEF425bla
Submitted by [http://2012.igem.org/Team:Potsdam_Bioware Potsdam_Bioware2012]
UP12 BBa K929100 vector.png






















The single chain fragment (scFV425) specifically binds to the epidermal growth factor receptor (EGFR) domain 3. The part has a shortened N-terminal FLAG-tag sequence of five amino acids (DYKDE) for purification and detection.

Characterization

For characterization and further applications see BBa_K929101.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 538
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None